The asthma biobank of Prof. Laprise comprises 1,448 DNA samples spread out in 254 extended families. Those French Canadian families are constituted of the proband, formerly recruited to the family members and the parents. Those are composed from 3 to 17 family members and the number of affected individuals among each of them varies from 1 to 10. The respiratory condition was evaluated following the criteria of the American Thoracic Society (metacholine test, forced expiratory volume in one second, serum IgE level, skin prick test, cell counts and several other data such as past medical reports, body mass index and medication). A Genome Wide Association Study (GWAS) was conducted with the Ilumina 610 K microarray and therefore the genotypes for more than 610 000 polymorphisms are available for 1,214 subjects. (Genes Immun. 2014 Mar 20)
A new recruiting process began in 2015 for 215 individuals included in the biobank, from which purified cell samples of eosinophils and naives CD4+ T lymphocytes were obtained. Methylation analysis by Methyl-capture sequencing and transcriptome analysis by sequencing were performed on both cell types. A new assessment of the condition of these participants (respiratory, skin prick test, medical history, etc.) is ongoing. The biobank now has longitudinal data for a group of the participants originally recruited.
More than 100 phenotypic data are available for the majority of the participants. For a complete description of the data, please feel free to contact Prof. Laprise.
The recruitment at the St-Justine Hospital at Montreal allows to regroup 40 children suffering of peanut allergy and their parents. The DNA of those 120 individuals is available to validate some genetic studies conducted by other researchers.
French Canadian Leigh Syndrome
French Canadian Leigh Syndrome Biobank
The LSFC biobank was established in 2008 in Prof. Laprise’s lab at the Univeristé du Québec à Chicoutimi. This biobank, globally unique, comprises sample from 21 affected individuals (of whom 7 came from therapeutic pregnancy termination (TPT)), 14 carriers and 23 controls of whom 3 came from TPT). Fibroblasts isolated from skin biopsies (primary and immortalized), DNA, RNA, tissues from TPT (cortez, brain, cerebellum, heart, kidney, liver, muscle, etc) are available for the researchers member of the consortium and also for any researcher for which the research project have been scientifically and ethically approved by the consortium. The recruitment is constant and additional individuals joined the biobank each year.
Epidermolysis bullosa simplex (EBS)
Epidermolysis bullosa simplex (EBS) Biobank
In 2008, a biobank for the research on EBS was established in Prof. Laprise’s lab at the Univeristé du Québec à Chicoutimi. This biobank is the largest one for EBS in the world and comprises samples from 9 EBS patients and their paired (age and sexe) controls. Keratinocytes cell lines (primary and immortalized) and fibroblasts isolated from skin biopsies, DNA and RNA is available.